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denaturing high-performance liquid chromatography (dhplc) wave 1100  (Transgenomic)

 
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    Transgenomic denaturing high-performance liquid chromatography (dhplc) wave 1100
    RT-PCR analysis of PCDH10 in human cancer cell lines. <t>dHPLC</t> analysis of RT-PCR amplified cDNA shows a peak indicating the expression of PCDH10 in the breast adenocarcinoma MB-231 cells, in line with the lack of methylation of the corresponding promoter in the same cells. Conversely, no peaks are detected in Capan-2 and AsPC-1 pancreatic adenocarcinoma and in AGS gastric adenocarcinoma cells, indicating lack of PCDH10 mRNA expression in these cell lines, whose PCDH10 promoter is fully methylated
    Denaturing High Performance Liquid Chromatography (Dhplc) Wave 1100, supplied by Transgenomic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/denaturing+high-performance+liquid+chromatography+%28dhplc%29+wave+1100/pmc06518703-173-7-13?v=Transgenomic
    Average 90 stars, based on 1 article reviews
    denaturing high-performance liquid chromatography (dhplc) wave 1100 - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "High methylation levels of PCDH10 predict poor prognosis in patients with pancreatic ductal adenocarcinoma"

    Article Title: High methylation levels of PCDH10 predict poor prognosis in patients with pancreatic ductal adenocarcinoma

    Journal: BMC Cancer

    doi: 10.1186/s12885-019-5616-2

    RT-PCR analysis of PCDH10 in human cancer cell lines. dHPLC analysis of RT-PCR amplified cDNA shows a peak indicating the expression of PCDH10 in the breast adenocarcinoma MB-231 cells, in line with the lack of methylation of the corresponding promoter in the same cells. Conversely, no peaks are detected in Capan-2 and AsPC-1 pancreatic adenocarcinoma and in AGS gastric adenocarcinoma cells, indicating lack of PCDH10 mRNA expression in these cell lines, whose PCDH10 promoter is fully methylated
    Figure Legend Snippet: RT-PCR analysis of PCDH10 in human cancer cell lines. dHPLC analysis of RT-PCR amplified cDNA shows a peak indicating the expression of PCDH10 in the breast adenocarcinoma MB-231 cells, in line with the lack of methylation of the corresponding promoter in the same cells. Conversely, no peaks are detected in Capan-2 and AsPC-1 pancreatic adenocarcinoma and in AGS gastric adenocarcinoma cells, indicating lack of PCDH10 mRNA expression in these cell lines, whose PCDH10 promoter is fully methylated

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Amplification, Expressing, Methylation



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    denaturing high-performance liquid chromatography (dhplc) wave 1100  (Transgenomic)
    90
    Transgenomic denaturing high-performance liquid chromatography (dhplc) wave 1100
    RT-PCR analysis of PCDH10 in human cancer cell lines. <t>dHPLC</t> analysis of RT-PCR amplified cDNA shows a peak indicating the expression of PCDH10 in the breast adenocarcinoma MB-231 cells, in line with the lack of methylation of the corresponding promoter in the same cells. Conversely, no peaks are detected in Capan-2 and AsPC-1 pancreatic adenocarcinoma and in AGS gastric adenocarcinoma cells, indicating lack of PCDH10 mRNA expression in these cell lines, whose PCDH10 promoter is fully methylated
    Denaturing High Performance Liquid Chromatography (Dhplc) Wave 1100, supplied by Transgenomic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/denaturing+high-performance+liquid+chromatography+%28dhplc%29+wave+1100/pmc06518703-173-7-13?v=Transgenomic
    Average 90 stars, based on 1 article reviews
    denaturing high-performance liquid chromatography (dhplc) wave 1100 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    RT-PCR analysis of PCDH10 in human cancer cell lines. dHPLC analysis of RT-PCR amplified cDNA shows a peak indicating the expression of PCDH10 in the breast adenocarcinoma MB-231 cells, in line with the lack of methylation of the corresponding promoter in the same cells. Conversely, no peaks are detected in Capan-2 and AsPC-1 pancreatic adenocarcinoma and in AGS gastric adenocarcinoma cells, indicating lack of PCDH10 mRNA expression in these cell lines, whose PCDH10 promoter is fully methylated

    Journal: BMC Cancer

    Article Title: High methylation levels of PCDH10 predict poor prognosis in patients with pancreatic ductal adenocarcinoma

    doi: 10.1186/s12885-019-5616-2

    Figure Lengend Snippet: RT-PCR analysis of PCDH10 in human cancer cell lines. dHPLC analysis of RT-PCR amplified cDNA shows a peak indicating the expression of PCDH10 in the breast adenocarcinoma MB-231 cells, in line with the lack of methylation of the corresponding promoter in the same cells. Conversely, no peaks are detected in Capan-2 and AsPC-1 pancreatic adenocarcinoma and in AGS gastric adenocarcinoma cells, indicating lack of PCDH10 mRNA expression in these cell lines, whose PCDH10 promoter is fully methylated

    Article Snippet: The DNA digests were separated by denaturing high-performance liquid chromatography (dHPLC) (Wave 1100, Transgenomic, Omaha, NE).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Expressing, Methylation